Artificial Cells Capable of NO Generation with Light Controllable Membraneless Organelles for Melanoma Therapy
Abstract
Abstract Membraneless organelles (MLOs) formed by liquid–liquid phase separation exhibit diverse important biofunctions in cells. The construction of artificial cells containing MLOs with enhanced complexity and functions is still challenging. Here a light‐responsive protein, Cry2olig‐IDRs, is designed and purified to form MLOs upon light (488 nm) irradiation. They are capable of rapidly recruiting positively charged inducible nitric oxide synthase (iNOS + ) from surroundings to regulate its activity for NO production. NO‐artificial cells are constructed by encapsulating Cry2olig‐IDRs and iNOS + into giant unilamellar vesicles, which are capable of rapid production of NO with high concentration due to the formation of MLOs upon light irradiation. NO‐artificial cells are confirmed to possess the ability for melanoma tumor therapy in mice. These findings provide an efficient method for remotely regulating enzyme activity inside artificial cells, paving the path to build more sophisticated artificial cells for their biomedical applications.
Article Details
Authors (12)
Yingming Zhao
Shubin Li
State Key Laboratory of Urban-Rural Water Resource and Environment, MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, Heilongjiang Provincial Joint Laboratory of Molecular Science (International Cooperation), School of Chemistry and Chemical Engineering
Yanhao Liu
Chao Li
Jingjing Zhao
Yongshuo Ren
State Key Laboratory of Urban-Rural Water Resource and Environment, MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, Heilongjiang Provincial Joint Laboratory of Molecular Science (International Cooperation), School of Chemistry and Chemical Engineering
Wan Zhao
Xiangxiang Zhang
Xinyu Cui
Xuefeng Tang
State Key Laboratory of Urban Water Resource and Environment MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical Engineering Harbin Institute of Technology No. 92, West Da‐Zhi Street Harbin 150001 China
Peipei Ren
State Key Laboratory of Urban Water Resource and Environment MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical Engineering Harbin Institute of Technology No. 92, West Da‐Zhi Street Harbin 150001 China
Xiaojun Han
State Key Laboratory of Urban-Rural Water Resource and Environment, MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, Heilongjiang Provincial Joint Laboratory of Molecular Science (International Cooperation), School of Chemistry and Chemical Engineering