Charge‐Driven Bioshield Remodels Diabetic Oral Microenvironment for Accelerated Wound Healing
Abstract
ABSTRACT Diabetic chronic wounds, especially in the moist and mechanically dynamic oral environment, pose a significant therapeutic challenge due to neutrophil extracellular traps (NETs)‐mediated inflammation, biofilm infections, and extracellular matrix remodeling defects. To address this challenge, we develop a protein‐based adhesive bioshield, functioning through the combined action of physical blocking and electrostatic interaction, that simultaneously acts as a bacterial barrier and NETs scavenger, thereby reactivating the focal adhesion signaling. The protein's high‐density lysine residues establish a robust adhesion network that resists the dynamic oral environment while creating a persistent antibacterial bioshield. Notably, the high net charge of adhesive bioshield enables rapid NETs neutralization by electrostatically binding cell‐free DNA (cfDNA), which reduces local NETs levels and inflammatory responses. This NETs clearance subsequently alleviates inflammation‐mediated suppression of focal adhesion signaling, which coordinately accelerates diabetic wound healing through enhanced epithelial migration, endothelial proliferation, and angiogenesis. Overall, this study presents a charge‐driven therapeutic strategy that targets NETs‐mediated inflammation, offering an alternative approach for managing diabetic wounds in dynamic and wet environments.
Article Details
Authors (13)
Yajuan Xie
Department of Orthodontics National Center for Stomatology National Clinical Research Center For Oral Diseases National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing Key Laboratory of Digital Stomatology Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health NMPA Key Laboratory for Dental Materials Peking University School and Hospital of Stomatology Beijing China
Yunfan Zhang
Yue Yang
Xiaomo Liu
Department of Orthodontics National Center for Stomatology National Clinical Research Center For Oral Diseases National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing Key Laboratory of Digital Stomatology Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health NMPA Key Laboratory for Dental Materials Peking University School and Hospital of Stomatology Beijing China
Ruichu Zhang
Department of Orthodontics National Center for Stomatology National Clinical Research Center For Oral Diseases National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing Key Laboratory of Digital Stomatology Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health NMPA Key Laboratory for Dental Materials Peking University School and Hospital of Stomatology Beijing China
Yabo Wang
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering
Bo Li
Chao Ma
Hongjie Zhang
State Key Laboratory of Rare Earths
Yusai Zhou
Engineering Research Center of Advanced Rare Earth Materials (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China
Bing Han
Kai Liu
Yan Wei
National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Laboratory of Biomedical Materials