Derivation of Mesenchymal Stem Cells from Induced Pluripotent Stem Cells and Their Application in Genetic Disease Modeling 2307485
Abstract
Abstract Introduction Thymic stromal cells are essential for T-cell progenitor proliferation and differentiation. While thymic epithelial cells are well studied, non-epithelial stromal populations–particularly neural crest—derived mesenchymal cells–are increasingly recognized for their roles in thymus formation and function. Mesenchymal defects contribute to thymic abnormalities in congenital syndromes such as DiGeorge syndrome (DGS), CHARGE syndrome, and Trisomy 21 (T21), yet human studies are limited by the rarity of these conditions and restricted access to thymic tissue. Methods We generated iPSCs from individuals with TBX1, CHD7, HOXA3, and PAX1 mutations, as well as DGS and T21 patients. Control and patient iPSCs, derived from PBMCs or skin biopsies, were differentiated into mesenchymal stem cells (MSCs). Primary thymic mesenchymal cells (ThyMCs) were also isolated from human thymi. All cell populations were analyzed by flow cytometry and bulk RNA-seq. Tri-lineage differentiation assays (adipogenic, chondrogenic, osteogenic) were performed to assess functional pathway defects. Results Flow cytometry confirmed robust expression of MSC markers (CD73, CD146, CD105) in all MSC and ThyMC samples, absent in undifferentiated iPSCs. Principal component analysis revealed clear segregation of iPSCs, MSCs, and ThyMCs. Transcriptomic profiling showed that all patient-derived MSCs acquired mesenchymal identity, but each disease group displayed distinct transcriptional changes. MSCs with TBX1, HOXA3, or PAX1 mutations had the highest number of differentially expressed genes, affecting pathways such as extracellular matrix and cartilage development.DGS-derived MSCs and ThyMCs showed marked upregulation of ECM and collagen genes (FBLN5, PCOLCE, EMILIN1, COL3A1, COL1A2). Conclusion Our findings reveal disease-specific mesenchymal defects underlying thymic abnormalities in congenital syndromes.This platform enables mechanistic studies of thymic stromal dysfunction and advances understanding of immune deficits in these disorders. Funding Source This work was supported by the Division of Intramural Research, NIAID, NIH. Topic Categories Hematopoiesis and Immune System Development (HEM)
Article Details
Journal Info
The Journal of Immunology
American Association of Immunologists
Authors (16)
Giuseppe Sangiorgio
Division of Cardiology, Azienda Ospedaliero-Universitaria Policlinico “G. Rodolico–San Marco,” University of Catania, Italy.
Francesca Pala
Laboratory of Clinical Immunology and Microbiology, IDGS, NIAID, NIH , Bethesda, MD,
Kayla Amini
Laboratory of Clinical Immunology and Microbiology, IDGS, NIAID, NIH , Bethesda, MD,
Eduardo Anaya
Laboratory of Clinical Immunology and Microbiology, IDGS, NIAID, NIH , Bethesda, MD,
Sarah Dinges
Laboratory of Clinical Immunology and Microbiology, IDGS, NIAID, NIH, Bethesda, MD, USA; Department of Paediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité - Universitätsmedizin Berlin , Berlin,
Ottavia M Delmonte
Laboratory of Clinical Immunology and Microbiology, IDGS, NIAID, NIH , Bethesda, MD,
Alexandra Freeman
Laboratory of Clinical Immunology and Microbiology, IDGS, NIAID, NIH , Bethesda, MD,
Alexandra Y Kreins
UCL Great Ormond Street Institute of Child Health and Department of Immunology and Gene Therapy, Great Ormond Street Hospital for Children NHS Foundation Trust , London,
E Graham Davies
Infection, Immunity and Inflammation Theme, UCL Great Ormond Street Institute of Child Health, London, United Kingdom; Department of Immunology, Great Ormond Street Hospital , London,
Horst von Bernuth
Cathleen Collins
Division of Allergy Immunology, Department of Pediatrics, University of California, San Diego , La Jolla, CA,
Maria Teresa de la Morena
Division of Immunology, Department of Pediatrics, Seattle Children’s Hospital, University of Washington , Seattle, WA,
Melinda M Rathkopf
Director, Allergy, Asthma & Immunology Center of Alaska , Anchorage, AK,
Dusan Bogunovic
Marita Bosticardo
Luigi D Notarangelo
Laboratory of Clinical Immunology and Microbiology, IDGS, NIAID, NIH , Bethesda, MD,