Innovative MESF Standards for Accurate Quantification of Biological Nanoparticles in Flow Cytometry 2306500

Abstract

Abstract Introduction The quantification of fluorescence intensity in flow cytometry is important for instrument sensitivity characterization and standardization of results. One of the key tools in this regard is Molecules of Equivalent Soluble Fluorochrome (MESF) beads. These beads are essential for standardizing fluorescence measurements and ensuring consistency across different experiments and instruments. Methods The quantification of fluorescence intensity in flow cytometry is important for instrument sensitivity characterization and standardization of results. One of the key tools in this regard is Molecules of Equivalent Soluble Fluorochrome (MESF) beads. These beads are essential for standardizing fluorescence measurements and ensuring consistency across different experiments and instruments. Results Preliminary evaluation using the CytoFLEX nano Flow Cytometer has confirmed the potential utility of the new nano-sized MESF beads Conclusion Once fully developed, these beads are anticipated to become an essential tool for MESF characterization and for standardizing instruments and assays in the growing field of small particle flow cytometry Funding Source n/a Topic Categories Technological Innovations in Immunology (TECH)