Multiphysics‐Driven Assembly of Biomimetic Vesicles
Abstract
ABSTRACT Artificial extracellular vesicles (AEVs) are programmable, biomimetic materials that combine the structural and biological complexity of naturally secreted extracellular vesicles (NEVs) with the design flexibility of synthetic nanomaterials. A multiphysics‐driven microfluidics is developed to efficiently integrate the nanoknife‐assisted membrane rupture with flow dynamics and acoustothermal modulation for the reproducible, high‐yield, scalable, and standardized production of AEVs. Compared to empirical mechanical processes, this integrated microfluidic workflow, which exploits physical and biological insights for EV production, enables multiphysics‐based predictions for a precise control of material inputs, flow dynamics, and cell‐knife interactions within the channel. The biomimetic AEVs developed through this integrated, optimized single‐flow platform, with a sustained and efficient therapeutic encapsulation process, preserve native protein architectures to conduct biomimetic mechanisms of immune modulation and homologous targeting. The standardizable microfluidic platform paves the way for a structure–process–function design strategy, enabling the formation of scalable, adaptive biomaterials for the development of bioinspired interfacial engineering and biomedicine.
Article Details
Authors (17)
Timofei Solodko
Heinz‐Nixdorf‐Chair of Biomedical Electronics School of Computation Information and Technology & Munich Institute of Biomedical Engineering Center for Translational Cancer Research (TranslaTUM) Technical University of Munich (TUM) Munich Germany
Ian Gimino
Heinz‐Nixdorf‐Chair of Biomedical Electronics School of Computation Information and Technology & Munich Institute of Biomedical Engineering Center for Translational Cancer Research (TranslaTUM) Technical University of Munich (TUM) Munich Germany
Aastha Chandiwala
Heinz‐Nixdorf‐Chair of Biomedical Electronics School of Computation Information and Technology & Munich Institute of Biomedical Engineering Center for Translational Cancer Research (TranslaTUM) Technical University of Munich (TUM) Munich Germany
Bayan Alkotoub
Chair of Biological Imaging TranslaTUM School of Medicine and Health & School of Computation Information and Technology TUM Munich Germany
Ali Bashiri Dezfouli
Radiation Immuno‐Oncology Group TranslaTUM TUM School of Medicine and Health University Hospital of TUM Munich Germany
Heiko Reith
Institute for Metallic Materials Leibniz Institute for Solid State and Materials Research Dresden Germany
Asjad Bakhtiar
Heinz‐Nixdorf‐Chair of Biomedical Electronics School of Computation Information and Technology & Munich Institute of Biomedical Engineering Center for Translational Cancer Research (TranslaTUM) Technical University of Munich (TUM) Munich Germany
Rojda Hicsanmaz
Heinz‐Nixdorf‐Chair of Biomedical Electronics School of Computation Information and Technology & Munich Institute of Biomedical Engineering Center for Translational Cancer Research (TranslaTUM) Technical University of Munich (TUM) Munich Germany
Johann Brenner
Research Group CryoEM Technology Max Planck Institute of Biochemistry Martinsried Germany
Christoph J. O. Kaiser
Research Group CryoEM Technology Max Planck Institute of Biochemistry Martinsried Germany
Elena Belli
Shilpi Pandey
Oregon Health and Science University
Samuel D. Rabkin
Department of Neurosurgery Massachusetts General Hospital and Harvard Medical School Boston Massachusetts USA
Kornelius Nielsch
Gabriele Multhoff
Radiation Immuno‐Oncology Group TranslaTUM TUM School of Medicine and Health University Hospital of TUM Munich Germany
Oliver Hayden
Heinz‐Nixdorf‐Chair of Biomedical Electronics School of Computation Information and Technology & Munich Institute of Biomedical Engineering Center for Translational Cancer Research (TranslaTUM) Technical University of Munich (TUM) Munich Germany
Morteza Hasanzadeh Kafshgari
Heinz‐Nixdorf‐Chair of Biomedical Electronics School of Computation Information and Technology & Munich Institute of Biomedical Engineering Center for Translational Cancer Research (TranslaTUM) Technical University of Munich (TUM) Munich Germany