Nanoscale Biodegradable Printing for Designed Tuneability of Vaccine Delivery Kinetics
Abstract
Abstract Two‐photon polymerization (2PP) 3D printing enables top–down biomaterial synthesis with nanoscale spatial resolution for de novo design of monodisperse injectable drug delivery systems. Spatiotemporal Controlled Release Inks of Biocompatible polyEsters (SCRIBE) is a novel poly(lactic‐ co ‐glycolic acid)‐triacrylate resin family with sub‐micron resolution and tuneable hydrolysis that addresses the limitations of current 2PP resins. SCRIBE enables the direct printing of hollow microparticles with tuneable chemistry and complex geometries inaccessible to molding techniques, which are used to engineer controlled protein release in vitro and in vivo. SCRIBE microparticles are used to modulate antibody titers and class switching as a function of antigen release rate and extend these findings to enable a single‐injection vaccine formulation with extended antibody induction kinetics. Demonstrating how the chemistry and computer‐aided design of 2PP‐printed microparticles can be used to tune responses to biomacromolecule release in vivo opens significant opportunities for a new generation of drug delivery vehicles.
Article Details
Authors (17)
David J. Peeler
Department of Materials Department of Bioengineering and Institute for Biomedical Engineering Imperial College London London UK
Rujie Sun
Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London South Kensington Campus London SW7 2AZ United Kingdom
Ceren Kütahya
Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London South Kensington Campus London SW7 2AZ United Kingdom
Patrick Peschke
Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London South Kensington Campus London SW7 2AZ United Kingdom
Kun Zhou
Key Laboratory of Animal Virology, Ministry of Agricultural and Rural Affairs of China and Zhejiang Provincial Engineering Research Center of Animal Biological Products, Department of Veterinary Medicine, Zhejiang University College of Animal Sciences
Giulia Brachi
Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London South Kensington Campus London SW7 2AZ United Kingdom
Jonathan Yeow
Omar Rifaie‐Graham
Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London South Kensington Campus London SW7 2AZ United Kingdom
Jonathan P. Wojciechowski
Department of Physiology, Anatomy and Genetics, Department of Engineering Science, Kavli Institute for Nanoscience Discovery, University of Oxford, Sherrington Rd, Oxford OX1 3QU, U.K.
Thomas F. F. Fernandez Debets
Department of Physiology, Anatomy and Genetics, Department of Engineering Science, Kavli Institute for Nanoscience Discovery, University of Oxford, Sherrington Rd, Oxford OX1 3QU, U.K.
Vernon LaLone
Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London South Kensington Campus London SW7 2AZ United Kingdom
Xin Song
Krunal Polra
Department of Infectious Disease Faculty of Medicine Imperial College London South Kensington Campus London SW7 2AZ United Kingdom
Paul F. McKay
Department of Infectious Disease Faculty of Medicine Imperial College London South Kensington Campus London SW7 2AZ United Kingdom
John S. Tregoning
Robin J. Shattock
Molly M. Stevens