RP1 Combined With Nivolumab in Advanced Anti–PD-1–Failed Melanoma (IGNYTE)

M Michael K. Wong M Mohammed M. Milhem (Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA) J Joseph J. Sacco J Judith Michels (Département de Médecine Oncologique, Gustave Roussy, Villejuif, France) G Gino K. In E Eva Muñoz Couselo (Vall d’Hebron Institute of Oncology (VHIO) and Vall d’Hebron Hospital Medical Oncology Department, Barcelona, Spain) D Dirk Schadendorf G Georgia M. Beasley (Duke Cancer Institute, Duke University, Durham, NC) J Jiaxin Niu (Banner MD Anderson Cancer Center, Gilbert, AZ) B Bartosz Chmielowski T Trisha M. Wise-Draper T Tawnya Lynn Bowles (Intermountain Medical Center, Murray, UT) K Katy K. Tsai C Céleste Lebbé C Caroline Gaudy-Marqueste (From the Sandra and Edward Meyer Cancer Center (J.D.W.) and the Department of Medicine (J.D.W., M.A.P.), Weill Cornell Medicine, and Memorial Sloan Kettering Cancer Center (M.A.P.) — both in New York; Istituto Oncologico Veneto, IRCCS, Padua (V.C.-S.), European Institute of Oncology, IRCCS, Milan (P.Q.), Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori, IRCCS, Meldola (M.G.), University of Siena and the Center for Immuno-Oncology, University Hospital of Siena, Siena (M.M.), and Istituto Nazionale Tumori IRCCS Fondazione Pascale, Naples (P.A.A.) — all in Italy; Maria Sklodowska-Curie National Institute of Oncology, Warsaw, Poland (P.R.); Texas Oncology–Baylor Charles A. Sammons Cancer Center, Dallas (C.L.C.); University Hospital Essen, the German Cancer Consortium, the National Center for Tumor Diseases–West, the Research Alliance Ruhr, Research Center One Health, and University Duisburg-Essen — all in Essen, Germany (D.S.); the College of Medicine, Swansea University, Swansea (J.W.), Brist...) M Mark R. Middleton A Aglaia Skolariki (Churchill Hospital and University of Oxford, Oxford, United Kingdom) A Adel Samson (Leeds Institute of Medical Research at St. James’s, University of Leeds, Leeds, United Kingdom) J Jason A. Chesney (James Graham Brown Cancer Center, University of Louisville, Louisville, KY) A Ari M. VanderWalde (West Cancer Center and Research Institute, Germantown, TN) Y Yousef Zakharia (Division of Hematology and Medical Oncology, Department of Internal Medicine Mayo Clinic Phoenix Arizona USA) K Kevin J. Harrington (Institute of Cancer Research, Royal Marsden Hospital, London) E Elizabeth Appleton P Praveen K. Bommareddy (Replimune, Inc, Woburn, MA) J Junhong Zhu (Replimune, Inc, Woburn, MA) M Marcus Viana (Replimune, Inc., Woburn, MA) J Jeannie W. Hou (Replimune, Inc, Woburn, MA) R Robert S. Coffin (Replimune, Inc, Woburn, MA) C Caroline Robert

Abstract

PURPOSE Effective treatment options for melanoma after immune checkpoint blockade failure are limited. RP1 (vusolimogene oderparepvec) is a herpes simplex virus type 1–based oncolytic immunotherapy, here evaluated in combination with nivolumab in anti–PD-1–failed melanoma. METHODS Patients had advanced melanoma that had confirmed progression on anti–PD-1 (≥8 weeks, last prior treatment). RP1 was administered intratumorally (≤8 doses, ≤10 mL/dose; additional doses allowed) with nivolumab (≤2 years). The objective response rate (ORR) was assessed by independent central review using Response Evaluation Criteria in Solid Tumors version 1.1. RESULTS Of 140 patients enrolled, 48.6% had stage IVM1b/c/d disease, 65.7% had primary anti–PD-1 resistance, 56.4% were PD-L1 negative, and 46.4% received prior anti–PD-1 and anti–cytotoxic T-lymphocyte antigen-4 therapy (43.6% in combination and 2.9% sequentially). Confirmed ORR (95% CI) was 32.9% (95% CI, 25.2% to 41.3%; 15.0% complete response). Responses occurred with similar frequency, depth, duration, and kinetics for injected and noninjected, including visceral lesions. The median (95% CI) duration of response was 33.7 (95% CI, 14.1 to not reached) months. Overall survival rates (95% CI) at 1 and 2 years were 75.3% (95% CI, 66.9% to 81.9%) and 63.3% (95% CI, 53.6% to 71.5%), respectively. Biomarker analysis demonstrated broad immune activation associated with response, including increased CD8 + T-cell infiltration and PD-L1 expression. Treatment-related adverse event rates were 77.1% grade 1/2, 9.3% grade 3, 3.6% grade 4, and no grade 5 events. CONCLUSION RP1 combined with nivolumab provided deep and durable systemic responses in patients with anti–PD-1–failed melanoma, including those with poor prognostic factors. The safety profile was favorable, with mostly grade 1/2 adverse events.

Article Details

Volume / Issue Vol. 43, Issue 33
Published November 20, 2025
Pages 3589-3599
ISSN 0732-183X
Publisher Lippincott Williams & Wilkins

Journal Info

Journal of Clinical Oncology

Lippincott Williams & Wilkins

ISSN: 0732-183X Health Sciences

Authors (29)

M

Michael K. Wong

M

Mohammed M. Milhem

Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA

J

Joseph J. Sacco

J

Judith Michels

Département de Médecine Oncologique, Gustave Roussy, Villejuif, France

G

Gino K. In

E

Eva Muñoz Couselo

Vall d’Hebron Institute of Oncology (VHIO) and Vall d’Hebron Hospital Medical Oncology Department, Barcelona, Spain

D

Dirk Schadendorf

G

Georgia M. Beasley

Duke Cancer Institute, Duke University, Durham, NC

J

Jiaxin Niu

Banner MD Anderson Cancer Center, Gilbert, AZ

B

Bartosz Chmielowski

T

Trisha M. Wise-Draper

T

Tawnya Lynn Bowles

Intermountain Medical Center, Murray, UT

K

Katy K. Tsai

C

Céleste Lebbé

C

Caroline Gaudy-Marqueste

From the Sandra and Edward Meyer Cancer Center (J.D.W.) and the Department of Medicine (J.D.W., M.A.P.), Weill Cornell Medicine, and Memorial Sloan Kettering Cancer Center (M.A.P.) — both in New York; Istituto Oncologico Veneto, IRCCS, Padua (V.C.-S.), European Institute of Oncology, IRCCS, Milan (P.Q.), Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori, IRCCS, Meldola (M.G.), University of Siena and the Center for Immuno-Oncology, University Hospital of Siena, Siena (M.M.), and Istituto Nazionale Tumori IRCCS Fondazione Pascale, Naples (P.A.A.) — all in Italy; Maria Sklodowska-Curie National Institute of Oncology, Warsaw, Poland (P.R.); Texas Oncology–Baylor Charles A. Sammons Cancer Center, Dallas (C.L.C.); University Hospital Essen, the German Cancer Consortium, the National Center for Tumor Diseases–West, the Research Alliance Ruhr, Research Center One Health, and University Duisburg-Essen — all in Essen, Germany (D.S.); the College of Medicine, Swansea University, Swansea (J.W.), Brist...

M

Mark R. Middleton

A

Aglaia Skolariki

Churchill Hospital and University of Oxford, Oxford, United Kingdom

A

Adel Samson

Leeds Institute of Medical Research at St. James’s, University of Leeds, Leeds, United Kingdom

J

Jason A. Chesney

James Graham Brown Cancer Center, University of Louisville, Louisville, KY

A

Ari M. VanderWalde

West Cancer Center and Research Institute, Germantown, TN

Y

Yousef Zakharia

Division of Hematology and Medical Oncology, Department of Internal Medicine Mayo Clinic Phoenix Arizona USA

K

Kevin J. Harrington

Institute of Cancer Research, Royal Marsden Hospital, London

E

Elizabeth Appleton

P

Praveen K. Bommareddy

Replimune, Inc, Woburn, MA

J

Junhong Zhu

Replimune, Inc, Woburn, MA

M

Marcus Viana

Replimune, Inc., Woburn, MA

J

Jeannie W. Hou

Replimune, Inc, Woburn, MA

R

Robert S. Coffin

Replimune, Inc, Woburn, MA

C

Caroline Robert